Seliwanoff's reagent
Seliwanoff's reagent is a reagent used to test for ketohexoses, named after Russian chemist Feodor Feodorovich Selivanov (1859–1938).[1][2] It is a solution of resorcinol in hydrochloric acid.
Contents
Reagent
The reagent is prepared by dissolving 1 g of resorcinol in 330 cm3 of concentrated hydrochloric acid, and diluting to 1 litre with water. The final solution is 9 mmol dm−3 in resorcinol and approximately 4 mol dm−3 in hydrochloric acid.
Test
A few drops of the solution to be tested are added to about 1 cm3 of the reagent in a test tube. The mixture is heated to 100 °C and kept at that temperature for about five minutes, then allowed to cool. The appearance of a cherry-red colouration indicates the presence of a ketohexose.
References
- ↑ Seliwanoff, Theodor Notiz über eine Fruchtzuckerreaction. Ber. Dtsch. Chem. Ges. 1887, 20 (1), 181–82. DOI: 10.1002/cber.18870200144.
- ↑ Attwood, Teresa K.; Cammack, Richard Oxford dictionary of biochemistry and molecular biology, 2nd ed.; Oxford University Press, 2006; p 608. ISBN 0198529171, <http://books.google.es/books?id=XpUjsqD7lFUC&pg=PA608>.
External links
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See also the corresponding article on Wikipedia. |
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